MENTORING, STUDENT SUPERVISION, AND LEADERSHIP
Synthetic biology and genetic circuit design (constructs and cloning: Golden gate, Gibson assembly), Synthetic
promoter library screening (SPECs), Pooled miRNA library screening (mir-ts, mir-sensors), Genome engineering,
Single-nucleus RNA sequencing library preparation, Single cell/nuclei transcriptomics (droplet-based),
Metabolomics, mRNA-based cellular reprogramming, Transfection, Viral (lentivirus, AAV) & Non-viral
(lipofectamine, poly-transfection) gene delivery methods, RT-qPCR, ddPCR.
Engineered heart tissues (EHTs) for cardiac arrhythmia disease modeling, Electrical stimulation of EHTs,
Contractile force measurement of EHTs, Optical mapping to record action potentials in EHTs, Bioreactor
expansion of hPSCs, Design of experiments (DoE), Liquid handling robotics (Opentrons®OT-2).
Engineered heart tissues (EHTs) for cardiac arrhythmia disease modeling, Electrical stimulation of EHTs,
Contractile force measurement of EHTs, Optical mapping to record action potentials in EHTs, Bioreactor
expansion of hPSCs, Design of experiments (DoE), Liquid handling robotics (Opentrons®OT-2).
Mammalian cell culture: Various types of stem cells: pluripotent (mouse and human), bone marrow
mesenchymal (rat and human), glioblastoma (human), and neural (mouse and human).
2D differentiation of hPSCs into cardiac, neural, and hepatocyte lineages.
3D cardiac, brain, and liver organoids from hPSCs (patient and healthy donors).
Reverse toggling of primed to naïve hPSCs, Multicolor flow cytometry, Fluorescence-activated cell sorting
(FACS), Single-nuclei isolation from fresh/frozen cardiac tissues (patient and healthy), Karyotyping.
R programming language, Construction of ordinary differential equation (ODE) models and simulations, Bulk
RNAseq analysis (quality assessment, sequence alignment, ranked gene lists, gene annotation enrichment,
and statistics), Single cell/nuclei RNAseq analysis (quality control, pre-processing (ambient RNA removal, cell
filtering with Cell Bender), doublet identification (DoubletFinder), anchor integration (Seurat), cell clustering
and annotation (Seurat), mapping to human cell atlas (HCA), Data visualization in R (ggplot2), NicheNet &
CellChat to infer intercellular communication, Ingenuity pathway analysis (IPA), DAVID Bioinformatics, Cell
Ranger, KEGG, and Gene Ontology. Statistical analysis software: Minitab for design of experiments (DoEs),
Prism. Flow cytometry analysis software: FlowJo & CytoFlow (multicolor).
R programming language, Construction of ordinary differential equation (ODE) models and simulations, Bulk
RNAseq analysis (quality assessment, sequence alignment, ranked gene lists, gene annotation enrichment,
and statistics), Single cell/nuclei RNAseq analysis (quality control, pre-processing (ambient RNA removal, cell
filtering with Cell Bender), doublet identification (DoubletFinder), anchor integration (Seurat), cell clustering
and annotation (Seurat), mapping to human cell atlas (HCA), Data visualization in R (ggplot2), NicheNet &
CellChat to infer intercellular communication, Ingenuity pathway analysis (IPA), DAVID Bioinformatics, Cell
Ranger, KEGG, and Gene Ontology. Statistical analysis software: Minitab for design of experiments (DoEs),
Prism. Flow cytometry analysis software: FlowJo & CytoFlow (multicolor).
Mentoring ,Student Supervision, And Leadership
Kate Huang. “Investigating titin-truncating variants and arrhythmia using atrial and ventricular iPSC
CMs”. Dept. of Medicine, UBC, Canada.
Mohsen Khosravi Maharlooei. “Diabetic wound healing with adipose-derived MSCs”.
Stem Cell Research Lab, Shiraz University of Medical Sciences, Iran.
Current position: Assistant Professor of Immunology, Mayo Clinic, Arizona, USA
Oraya Srimokl. “Decoding hypertrophic cardiomyopathy progression with single-cell transcriptomics”. School of Biomedical Engineering, UBC, Canada.
1. Golsa Razian. “Bioprocessing of hPSCs in bioreactors”.
of Biochemistry & Molecular Biology, University of Calgary, Canada.
2. Current position: Scientist at Serqet Therapeutic (former FL60), Cambridge,USA.
1. Jayashabari Shankar. “Inferring enhancers and gene networks in a cardiac fibrosis model”.
Dept. of Biomedical Engineering, MIT Synthetic Biology Center, Cambridge, USA.
2. Nicholas Duan. “CRISPR/Cas9 genome editing of mESCs”.
Dept. of Medicine, University of Calgary, Canada.
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